Description

MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals. This kit uses Poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The 2×Hifair™ miRNA RT buffer in the product contains all the raw materials and primers for miRNA Poly(A)-tailing reaction and reverse transcription reaction. Careful optimization ensures that Poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end.
This product is recommended to be used in conjunction with our company's Hieff™ miRNA Universal qPCR SYBR Master Mix (Cat#11171) for optimal experimental results.

Features

• High sensitivity detection rate: 10 pg RNA can be detected
• High product stability: remains unaffected at 37℃ for 7 days

Application

• First-strand cDNA is synthesized and can be used as a template for RT-PCR and real-time RT-qPCR.

Specifications

Final product type	cDNA (First-Strand)
PCR method	RT-PCR
Reaction format	Isolated fraction
Sample type	miRNA
Optimal reaction temperature	37℃
Reverse transcriptase	MMLV

Components

Components No.	Name	11148ES50 (50T)
11148-A	Hifair™ miRNA RT enzyme mix	87.5 μL
11148-B	2×Hifair™ miRNA RT buffer	250 μL
11148-C	RNase-free H2O	10 mL
11148-D	Universal Reverse Primer (10 μmol/L)	4 mL
11148-E	U6 Forward Primer (10 μmol/L)	500 μL
11148-F	U6 Reverse Primer (10 μmol/L)	500 μL

Storage

The product should be stored at -25°C~-15℃ for 1 year.

Figures

Figure1. High sensitivity detection rate: 10 pg RNA could be detected.

The synthesized hsa-miR-let-7e-5p (a) and 293T cell total RNA (b) were used as templates and diluted to the following gradients: 60 copies to 606 copies (6 gradients) and 10 pg-100 ng (5 gradients). All gradient cDNA was detected.