FuniCut™ PacI

Details:

Description

FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps and can be used to digest plasmid, genomic and viral DNA as well as PCR products.

Features

  • Rapid digestion: DNA was digested rapidly by an enzyme for 5-15 min
  • Universal buffer: one tube reaction, simplifying the process
  • Stringent quality control

Applications

  • Molecular cloning
  • Southern blotting
  • SNP
  • Southern blotting
  • Genotyping

Specifications

Enzyme PacI
Cutting Site 5'-TTAAT↓TAA-3'
3'-AAT↑TAATT-5'
Optimal Reaction Temperature 37℃
Sensitive to Heat Inactivation YES
Type IIs RE NO
Methylation Sensitivity Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, EcoKI Methylation-Sensitive, Not EcoBI Methylation-Sensitive
Star Activity 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity
Isoschizomers NO

Components

Components No. Name 15041ES25 (25 T)
15041-A FuniCut™ PacI 25 μL
15041-B 10× FuniCut™ Buffer 1 mL
15041-C 10× FuniCut™ Color Buffer 1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Please avoid repeated freeze-thaw.

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