Description

FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.

Features

• Rapid digestion: DNA was digested rapidly by an enzyme for 5-15 min
• Universal buffer: one tube reaction, simplifying the process
• Stringent quality control

Applications

• Molecular cloning
• Southern blotting
• SNP
• Southern blotting
• Genotyping

Specifications

Enzyme	NsiI
Cutting Site	5'-ATGCA↓T-3' 3'-T↑ACGTA-5'
Optimal Reaction Temperature	37℃
Sensitive to Heat Inactivation	YES
Type IIs RE	NO
Methylation Sensitivity	Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive
Star Activity	3 h incubation did not show star activity, and delayed enzyme digestion might show star activity
Isoschizomers	EcoT22I, Mph1103I, Zsp2I

Components

Components No.	Name	15044ES25 (25 T)
15044-A	FuniCut™ NsiI	25 μL
15044-B	10× FuniCut™ Buffer	1 mL
15044-C	10× FuniCut™ Color Buffer	1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Please avoid repeated freeze-thaw.