FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
|Optimal Reaction Temperature||37℃|
|Sensitive to Heat Inactivation||YES|
|Type IIs RE||YES|
|Methylation Sensitivity||CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive|
|Star Activity||3 h incubation did not show star activity, and delayed enzyme digestion might show star activity|
|Isoschizomers||BsmBI, BstGZ53I, Esp16I, Esp23I|
|Components No.||Name||15048ES30 (30 T)|
|15048-A||FuniCut™ Esp3I(BsmBI)||30 μL|
|15048-B||10× FuniCut™ Buffer||1 mL|
|15048-C||10× FuniCut™ Color Buffer||1 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Please avoid repeated freeze-thaw.