FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Features
Applications
Enzyme | BstBI |
Cutting Site | 5'-TT↓CGAA-3' 3'-AAGC↑TT-5' |
ptimal Reaction Temperature | 37℃ |
Sensitive to Heat Inactivation | YES |
Type IIs RE | NO |
Methylation Sensitivity | CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, Not EcoBI Methylation-Sensitive |
Star Activity | 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
Isoschizomers | AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI |
Components No. | Name | 15050ES60 (100 T) |
15050-A | FuniCut™ BstBI | 100 μL |
15050-B | 10× FuniCut™ Buffer | 1 mL |
15050-C | 10× FuniCut™ Color Buffer | 1 mL |
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Please avoid repeated freeze-thaw.
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