RNase A (10 mg/mL)

Details:

Description

Ribonuclease A (RNase A) is a single-stranded polypeptide containing 4 disulfide bonds with a molecular weight of about 13.7 kDa. RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Specifically, the cleavage recognizes the phosphodiester bond formed by the 5'-ribose of a nucleotide and the phosphate group on the 3'-ribose of the adjacent pyrimidine nucleotide, so that the 2', 3' - Cyclic phosphates are hydrolyzed to the corresponding 3'-nucleoside phosphates (eg, pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-PG).
RNase A is the most active in cleaving single-stranded RNA and is active in a variety of reaction conditions: at low salt concentrations (0 to 100 mM NaCl), it can be used to cleave single-stranded RNA, double-stranded RNA, and RNA strands in RNA-DNA hybrids. while at high salt concentrations (≥0.3 M), RNase A can specifically cleave single-stranded RNA.
RNase A is most used to remove RNA during the preparation of plasmid DNA or genomic DNA. Whether or not DNase is active during the preparation process can easily affect the reaction. The traditional method of boiling in a water bath can be used to inactivate DNase activity. This product does not contain DNase and protease and does not require heat treatment before use. In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis.
This product is supplied as a solution at a concentration of 10 mg/mL. Recommended working concentration is 1-100 μg/mL, depending on the type of application.

Applications

  • Removal of RNA during genomic DNA preparation
  • RNA enzyme protection analysis
  • RNA sequence analysis

Specifications

Appearance liquid
Quantity 1 mL
Product type RNase A

Components

Components No. Name 10405ES03
10405 RNase A (10 mg/mL) 1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Citations & References:

[1] Qiu J, Fan Q, Xu S, et al. A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction. Biomaterials. 2022;285:121541. doi:10.1016/j.biomaterials.2022.121541(IF:12.479)

[2] Dou R, Qian J, Wu W, et al. Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma. Cell Death Dis. 2021;12(2):206. Published 2021 Feb 24. doi:10.1038/s41419-021-03510-4(IF:8.469)

[3] Ma H, Shen L, Yang H, Gong H, Du X. Circular RNA circPSAP functions as an efficient miR-331-3p sponge to regulate proliferation, apoptosis and bortezomib sensitivity of human multiple myeloma cells by upregulating HDAC4. J Pharmacol Sci. 2022;149(1):27-36. doi:10.1016/j.jphs.2022.01.013(IF:3.337)

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