Enhanced ECL Chemiluminescent Substrate Kit

Details:

Description

Enhanced ECL Chemiluminescent Substrate Kit is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle of Enhanced ECL Chemiluminescent Substrate Kit is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.
This kit has a unique luminescent substrate system, reducing the exposure background while introducing a new oxidant greatly improves the stability of the kit, allowing it to be stable at room temperature for up to one year. In addition to X-ray, fluorescent CCD scans can also be directly used mainly for WB detection and chemiluminescence immune detection systems.

Features

  • With high sensitivity and a high signal-to-noise ratio, it can detect medium pick-grade antigens.
  • Shine quickly and brightly, the imprinted film band can be observed under a fluorescent lamp.
  • Especially suitable for detecting target abundance is higher, conventional protein samples.
  • New oxidant, stable at room temperature for up to one year.

Applications

  • Chemiluminescence ELISA 
  • Western Blot 
  • Dot Blot-DNA/RNA
  • Southern blot-DNA
  • Northern blot-RNA

Specifications

Recommended Antibody Concentrations primary antibody (liquid storage concentration 1mg/mL): 1:1000-1:4000 secondary antibody (liquid storage concentration 1mg/mL): 1:1000-1:4000
Signal Duration < 2 h 
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase) Substrate
Sensitivity medium pick-grade antigens

Components

Components No. Name 36222ES60(100 mL) 36222ES76(500 mL)
36222-A Enhanced ECL - Reagent A 50 mL 250 mL
36222-B Enhanced ECL - Reagent B 50 mL 250 mL

Storage

The product should be stored at room temperature for one year. If not used for a long time, it is recommended to store at 2℃~8℃ to extend the validity period.
Notes: 36222-A should be stored away from the light!

Citations & References:

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