Product Description

Caerulein is a gastric regulatory molecule similar in function and composition to cholecystokinin (CCK), which stimulates gastric, bile duct and pancreatic secretion. Cerulein can be used to study signal transduction pathways mediated by NF-κB up-regulated proteins such as intercellular adhesion molecule (ICAM-1), inflammation-related factors such as NADPH oxidase, and Janus kinase. It has been successfully used in the establishment of acute pancreatitis (AP) models in animals such as rats, mice, dogs, and Syrian hamsters ^[1-5]. The modeling mechanism is:

1) Up-regulation of pancreatic acinar by stimulating intracellular NF-KB Intracellular Intercellular Adhesion Molecule (ICAM-1) expression. Surface ICAM-1 in turn promotes neutrophil adhesion to acinar cells to enhance pancreatic inflammatory effects ^[6];

2) leads to acinar cell death and pancreatic edema by inducing dysregulated digestive enzyme secretion and cytoplasmic vacuolation, thereby inducing pancreatitis;

3) activating inflammation-promoting factors ^[7].

Product Properties

English Synonym	Caerulein Sulfated, Cerulein, Ceruletide, [Tyr(SO3H) 4]Caerulein
Sequence	pGlu-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2
CAS NO.	17650-98-5
Purity	≥97%
Molecular Formular	C58H73N13O21S2
Molecular Weight	1352.4 g/moL
Appearance	White powder
Solubility	Soluble in DMSO at 2 mg/mL, insoluble in water

Shipping and Storage

The product is shipped with dry ice and the powder can be stored at -80°C for 2 years. Store at -20°C, valid for 1 year. The solution is stored at -80°C and has a validity period of 6 months. Store at -20°C, valid for 1 month.

Cautions

1)The cerulein powder has the best storage stability. The solution will reduce the shelf life, and the storage solution is recommended to be used within two months.

2)LPS can synergize with cerulein to establish an animal model of pancreatitis ^[4].

3)For your safety and health, please wear a lab coat and disposable gloves for the operation. For research use only!

Preparation method

First, make up a stock solution with a concentration of 2 mg/mL in DMSO. Store the solution in aliquots at -80℃ or -20℃ to avoid repeated freezing and thawing. For in vivo experiments, it needs to be diluted with a buffer solution (such as PBS) to the required working solution concentration before injection into mice.

References:

[1] Sans MD, et al. Caerulein-induced acute pancreatitis inhibits protein synthesis through effects on eIF2B and eIF4F. Am J Physiol Gastrointest Liver Physiol. 285(3):G517-28 (2003).

[2] Gül M, et al. The Beneficial Effects of Pentoxifylline on Caerulein-Induced Acute Pancreatitis in Rats. Dig Dis Sci. 54(3):555-63 (2009).

[3] Sharif R, et al. Impact of toll-like receptor 4 on the severity of acute pancreatitis and pancreatitis-associated lung injury in mice. Gut. 58(6):813-9 (2009).

[4] Elder AS, et al. Evaluation Of Lung Injury In A Caerulein Rat Model Of Acute Pancreatitis Complicated With Lipopolysaccharide. Pancreatology. 12(3):240-7 (2012).

[5] Ding SP, et al. A mouse model of severe acute pancreatitis induced with caerulein and lipopolysaccharide. World J Gastroenterol. 9(3):584-9 (2003).

[6] Zaninovic V, et al. Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells. Am J Physiol Gastrointest Liver Physiol. 279(4):G666-76 (2000).

[7] Kim H. Cerulein Pancreatitis: Oxidative Stress, Inflammation, and Apoptosis. Gut Liver. 2(2):74-80 (2008).