D-Luciferin, Potassium Salt

Details:

Description

D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging Technology. The mechanism of action is that luciferin (substrate) can be oxidized to emit light in the presence of ATP and luciferase. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice.  

D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use.

Features

  • No radiation, almost harmless to living organisms.
  • Bioluminescence, no excitation light source.
  • So sensitive, you can detect it in a few hundred cells.
  • Good penetration, 3-4cm tissue depth can still be detected.
  • High signal-to-noise ratio, strong fluorescence signal and good anti-interference.

Applications

  • In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
  • To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
  • The localization and distribution of foreign cells in animals were detected.
  • The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
  • Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.

Specifications

English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt
CAS NO. 115144-35-9
Formula C11H7N2O3S2K
Molecular weight 318.42 g/mol
Appearance Light yellow powder
Solubility Soluble in water(60 mg/mL)

Components

Components No. Name 40902ES01 40902ES02 40902ES03 40902ES08
40902 D-Luciferin, Potassium Salt 100mg 500 mg 1g 5g

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.

Citations & References:

[1] Gu Y, Wang Y, He L, et al. Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Mol Cancer. 2021;20(1):132. Published 2021 Oct 14. doi:10.1186/s12943-021-01435-2(IF:27.401)

[2] Huang K, Liang Q, Zhou Y, et al. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer [published correction appears in Cell Metab. 2021 Jan 5;33(1):223]. Cell Metab. 2019;30(6):1107-1119.e8. doi:10.1016/j.cmet.2019.09.014(IF:22.415)

[3] Dong X, Cheng R, Zhu S, et al. A Heterojunction Structured WO<sub>2.9</sub>-WSe<sub>2</sub> Nanoradiosensitizer Increases Local Tumor Ablation and Checkpoint Blockade Immunotherapy upon Low Radiation Dose. ACS Nano. 2020;14(5):5400-5416. doi:10.1021/acsnano.9b08962(IF:14.588)

[4] Zheng DW, Gao F, Cheng Q, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11(1):1985. Published 2020 Apr 24. doi:10.1038/s41467-020-15927-0(IF:12.121)

[5] Hu J, Su H, Cao H, et al. AUXIN RESPONSE FACTOR7 integrates gibberellin and auxin signaling via interactions between DELLA and AUX/IAA proteins to regulate cambial activity in poplar. Plant Cell. 2022;34(7):2688-2707. doi:10.1093/plcell/koac107(IF:11.277)

[6] Qi P, Huang M, Hu X, et al. A Ralstonia solanacearum effector targets TGA transcription factors to subvert salicylic acid signaling. Plant Cell. 2022;34(5):1666-1683. doi:10.1093/plcell/koac015(IF:11.277)

[7] Huang X, Qiu M, Wang T, et al. Carrier-free multifunctional nanomedicine for intraperitoneal disseminated ovarian cancer therapy. J Nanobiotechnology. 2022;20(1):93. Published 2022 Feb 22. doi:10.1186/s12951-022-01300-4(IF:10.435)

[8] Tang Y, Lin S, Yin S, et al. In situ gas foaming based on magnesium particle degradation: A novel approach to fabricate injectable macroporous hydrogels. Biomaterials. 2020;232:119727. doi:10.1016/j.biomaterials.2019.119727(IF:10.273)

[9] Zhang X, Zheng S, Hu C, et al. Cancer-associated fibroblast-induced lncRNA UPK1A-AS1 confers platinum resistance in pancreatic cancer via efficient double-strand break repair. Oncogene. 2022;41(16):2372-2389. doi:10.1038/s41388-022-02253-6(IF:9.867)

[10] Qiao K, Liu Y, Xu Z, et al. RNA m6A methylation promotes the formation of vasculogenic mimicry in hepatocellular carcinoma via Hippo pathway. Angiogenesis. 2021;24(1):83-96. doi:10.1007/s10456-020-09744-8(IF:9.780)

[11] Wu Q, Kuang K, Lyu M, et al. Allosteric deactivation of PIFs and EIN3 by microproteins in light control of plant development. Proc Natl Acad Sci U S A. 2020;117(31):18858-18868. doi:10.1073/pnas.2002313117(IF:9.412)

[12] Meng DF, Sun R, Liu GY, et al. S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1. Oncogene. 2020;39(30):5307-5322. doi:10.1038/s41388-020-1363-8(IF:7.971)

[13] Gao Y, Wang J, Han H, et al. A nanoparticle-containing polycaprolactone implant for combating post-resection breast cancer recurrence. Nanoscale. 2021;13(34):14417-14425. Published 2021 Sep 2. doi:10.1039/d1nr04125h(IF:7.790)

[14] Jiang W, Li T, Guo J, et al. Bispecific c-Met/PD-L1 CAR-T Cells Have Enhanced Therapeutic Effects on Hepatocellular Carcinoma. Front Oncol. 2021;11:546586. Published 2021 Mar 10. doi:10.3389/fonc.2021.546586(IF:6.244)

[15] Zhou Y , Zhou C , Zou Y , et al. Multi pH-sensitive polymer-drug conjugate mixed micelles for efficient co-delivery of doxorubicin and curcumin to synergistically suppress tumor metastasis. Biomater Sci. 2020;8(18):5029-5046. doi:10.1039/d0bm00840k(IF:6.183)

[16] Zhao X, Liu X, Zhang P, et al. Injectable peptide hydrogel as intraperitoneal triptolide depot for the treatment of orthotopic hepatocellular carcinoma. Acta Pharm Sin B. 2019;9(5):1050-1060. doi:10.1016/j.apsb.2019.06.001(IF:5.808)

[17] Li M, Wang J, Yu Y, et al. Characterization of Mesenchymal Stem Cells Derived from Bisphosphonate-Related Osteonecrosis of the Jaw Patients' Gingiva. Stem Cell Rev Rep. 2022;18(1):378-394. doi:10.1007/s12015-021-10241-8(IF:5.739)

[18] Zhang X, Li Y, Ji J, et al. Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis. Cell Death Discov. 2021;7(1):271. Published 2021 Oct 2. doi:10.1038/s41420-021-00667-x(IF:5.241)

[19] Wu D, Lv J, Zhao R, et al. PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomark Res. 2020;8:3. Published 2020 Jan 28. doi:10.1186/s40364-020-0183-x(IF:4.866)

[20] Qiao K, Chen C, Liu H, Qin Y, Liu H. Pinin Induces Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma by Regulating m6A Modification. J Oncol. 2021;2021:7529164. Published 2021 Dec 7. doi:10.1155/2021/7529164(IF:4.375)

[21] Li K, Liu T, Chen J, Ni H, Li W. Survivin in breast cancer-derived exosomes activates fibroblasts by up-regulating SOD1, whose feedback promotes cancer proliferation and metastasis. J Biol Chem. 2020;295(40):13737-13752. doi:10.1074/jbc.RA120.013805(IF:4.238)

[22] Kong Y, Feng Z, Chen A, et al. The Natural Flavonoid Galangin Elicits Apoptosis, Pyroptosis, and Autophagy in Glioblastoma. Front Oncol. 2019;9:942. Published 2019 Sep 27. doi:10.3389/fonc.2019.00942(IF:4.137)

[23] Kong Y, Feng Z, Chen A, et al. The Natural Flavonoid Galangin Elicits Apoptosis, Pyroptosis, and Autophagy in Glioblastoma. Front Oncol. 2019;9:942. Published 2019 Sep 27. doi:10.3389/fonc.2019.00942(IF:4.137)

[24] Hu J, Wang Y, Yuan Y. Inhibitors of APE1 redox function effectively inhibit γ-herpesvirus replication in vitro and in vivo. Antiviral Res. 2021;185:104985. doi:10.1016/j.antiviral.2020.104985(IF:4.101)

[25] Li Q, Wei D, Feng F, et al. α2,6-linked sialic acid serves as a high-affinity receptor for cancer oncolytic virotherapy with Newcastle disease virus. J Cancer Res Clin Oncol. 2017;143(11):2171-2181. doi:10.1007/s00432-017-2470-y(IF:3.503)

[26] Han B, Jiang P, Liu W, et al. Role of Daucosterol Linoleate on Breast Cancer: Studies on Apoptosis and Metastasis. J Agric Food Chem. 2018;66(24):6031-6041. doi:10.1021/acs.jafc.8b01387(IF:3.412)

[27] Hu H, Zhang Z, Wang R, et al. BGC823 Cell Line with the Stable Expression of iRFP720 Retains Its Primary Properties with Promising Fluorescence Imaging Ability. DNA Cell Biol. 2020;39(5):900-908. doi:10.1089/dna.2019.5057(IF:3.314)

[28] Ni XR, Zhao YY, Cai HP, et al. Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models. J Neurooncol. 2020;148(2):245-258. doi:10.1007/s11060-020-03527-3(IF:3.267)

[29] Feng H, Tang J, Zhang P, Miao Y, Wu T, Cheng Z. Anti-adipogenic 18,19-seco-ursane stereoisomers and oleane-type saponins from Ilex cornuta leaves. Phytochemistry. 2020;175:112363. doi:10.1016/j.phytochem.2020.112363(IF:3.044)

[30] Zhao Y, Li T, Tian S, et al. Effective Inhibition of MYC-Amplified Group 3 Medulloblastoma Through Targeting EIF4A1. Cancer Manag Res. 2020;12:12473-12485. Published 2020 Dec 3. doi:10.2147/CMAR.S278844(IF:2.886)

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