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0Hygromycin B (50 mg/mL)
Description
Hygromycin B, an aminoglycoside antibiotic synthesized by Streptomyces hygroscopicus, inhibits protein synthesis by interfering with 70S ribosomal translocation and inducing misreading of mRNA template, thus killing prokaryotes (such as bacteria), eukaryotes (such as yeasts, fungi) and higher mammalian eukaryotes.
Hygromycin resistance genes (hyg or hph) derived from Escherichia coli encode hygromycin B phosphotransferase, which detoxifies hygromycin B into a non-biologically active phosphorylated product, making it an excellent selective marker for screening and culturing prokaryotic or eukaryotic cells successfully transfected with hytromycin resistance genes. In addition, due to different modes of action, Hygromycin B is often used in combination with G418 or Blasticidin S for the selection of dual-resistant cell lines. Hygromycin B can also be used as an antiviral agent because it selectively penetrates into cells with increased membrane permeability due to viral infection and inhibits translation. It can also act as an insect-repellant via mixed with animal feed.
This product is a sterile hygromycin B solution (50 mg/mL in PBS buffer) and can be directly diluted for use with a culture medium.
Features
- High-quality raw materials, all antibiotic product raw materials are sourced from high-quality fixed suppliers
- Standardized production, using factory mass production mode
- Wide range of applications, which can be used in the fields of molecular biology and biochemical experimental research of tissue culture
- The cooperation platform covers the whole
- To ensure product quality stability, the difference between batches is controlled within 1%
Applications
- Screening of stably transfected cell lines
Specifications
| CAS No.
|
31282-04-9 |
| Molecular formula | C20H37N3O13 |
| Molecular weight | 527.52 g/mol |
| Purity | >90% (HPLC) |
| Potency | ≥1050 U/mg |
| Structure |
|
Components
| Components No. | Name | 60224ES03 | 60224ES10 |
| 60224 | Hygromycin B (50 mg/mL) | 1 g (20 mL) | 10*1 g (20 mL) |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Aliquot the solution and avoid repeated freezing and thawing.
Figure
Figure 1 Colony growth of E. coli on hygromycin-resistant plates with different concentrations, Yeasen and brand S* has the same effect
Experimental strain: E. coli
Note: A-C are brand S* hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates, D-F are Yeasen hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates
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