Description

Hifair™Ⅱ1st Strand cDNA Synthesis Kit (gDNA Digest plus) is a cDNA first strand synthesis kit containing gDNA removal steps. This kit is based on Hifair™ II Reverse Transcriptase. The thermostability of this enzyme has been greatly improved, and it can withstand the reaction temperature up to 50℃. It is suitable for the reverse transcription of RNA templates with complex secondary structures. At the same time, the enzyme enhances the affinity with the template and is suitable for reverse transcription of a small number of templates and low-copy genes. Hifair™Ⅱ The ability of Reverse Transcriptase to synthesize full-length cDNA has also been improved, which can expand to 10 kb of cDNA.

The kit contains a gDNA digester, which can remove the genomic DNA contamination left in the RNA template and ensure more reliable follow-up results. The kit provides two kinds of cDNA synthesis primers: Random Primers N6 and oligo (dT) 18. Users can select Random Primers N6, Oligo (dT) 18, or Gene Specific Primers as reverse transcription primers as required. The synthesized single-strand cDNA products can be directly used for subsequent PCR or qPCR reactions.

Features

• Efficiently synthesize full-length first-strand cDNAs up to 10kb in length.
• Complete -All components required for the RT reaction are provided.

Applications

• First-strand cDNA is synthesized and can be used as a template for RT-PCR and real-time RT-qPCR.
• Antisense RNA synthesis.

Specifications

Final product type	cDNA (First-Strand)
PCR method	RT-PCR
Reaction Format	Isolated fraction
Sample type	RNA
Optimal reaction temperature	42℃~50℃
Reverse transcriptase	MMLV

Components

Components No.	Name	11121ES60 (100T)
11121-A	RNase-free H2O	2×1 mL
11121-B	5×gDNA digester Buffer	200 μL
11121-C	gDNA digester	100 μL
11121-D	5×HifairTM Ⅱ Buffer plus	400 μL
11121-E	HifairTM Ⅱ Enzyme Mix	200 μL
11121-F	Oligo (dT)18 (50 μM)	100 μL
11121-G	Random Primers N6 (50μM)	100 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 18 months.