Anti-DYKDDDDK (Flag) Affinity Gel

Details:

Description

Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with Sepharose 4B gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.
Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Features

  • Specific—highly specific monoclonal antibody
  • High yield and high purity
  • Versatile—It can be used for protein purification or COIP
  • Convenient—reagents and peptides to elute are available separately

Applications

  • Immunoprecipitation (IP)
  • Purification of fusion proteins with Flag labels.

Specifications

Clone 1E6
Isotype Mouse IgG2b
Purity Protein A
Antibody Concentration 7.5 g antibody/L Gel
Protein binding capacity ≥1.1 mg protein/mL Gel
Storage buffer 10 mM Na3PO4, 150 mM NaCl, 50% glycerin, pH 7.4, contains 0.02% (w/v) sodium azide

Components

Components No. Name 20585ES01 20585ES03 20585ES08 20585ES25 20585ES60
20585 Anti-DYKDDDDK (Flag) Affinity Gel 100 μL 1 mL 5 mL 25 mL 100 mL

Storage

The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!

Figures

  • The SDS-PAGE gel electrophoresis resultfromof Protein purification with YEASEN Anti-Flag Affinity Gel.

Figure 1. The SDS-PAGE gel electrophoresis results from Protein purification with YEASEN Anti-Flag Affinity Gel.

1: electrophoresis bands were purified by adding enterobacter lysate
2: negative control

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