Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with Sepharose 4B gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.
Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).
Features
Applications
Clone | 1E6 |
Isotype | Mouse IgG2b |
Purity | Protein A |
Antibody Concentration | 7.5 g antibody/L Gel |
Protein binding capacity | ≥1.1 mg protein/mL Gel |
Storage buffer | 10 mM Na3PO4, 150 mM NaCl, 50% glycerin, pH 7.4, contains 0.02% (w/v) sodium azide |
Components No. | Name | 20585ES01 | 20585ES03 | 20585ES08 | 20585ES25 | 20585ES60 |
20585 | Anti-DYKDDDDK (Flag) Affinity Gel | 100 μL | 1 mL | 5 mL | 25 mL | 100 mL |
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!
Figure 1. The SDS-PAGE gel electrophoresis results from Protein purification with YEASEN Anti-Flag Affinity Gel.
1: electrophoresis bands were purified by adding enterobacter lysate
2: negative control
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