Heat-Labile UDG (uracil DNA glycosylase) excises uracil from dU-containing DNA by catalysing and hydrolysing uracil bases and N-glycoside bonds of the sugar phosphate skeletons, releasing free uracil. Compared with ordinary UDG enzyme, Heat-Labile UDG can avoid the degradation of dU-containing amplified products at room temperature by residual activity of ordinary UDG. The product works at room temperature and is sensitive to temperature and easy to be inactivated.
Remove aerosol pollution of dU-containing PCR products.
Remove uracil bases of single or double-stranded DNA.
10303ES60 (100 U)
10303ES76 (500 U)
10303ES92 (10000 U)
Uracil DNA Glycosylase (UDG), Heat-Labile (1 U/μL)
One unit (U) is the amount of enzyme that required to liberate 1 μg uracil from dU-containing DNA in 30 minutes at 25°C.
50℃, 10 min.
Dry ice transportation. Store at -20℃.
For your safety and health, please wear experimental clothes and disposable gloves.
Heat-Labile UDG was found to be active in most PCR buffers.
Endonuclease residue detection: DNA electrophoresis bands do not change when 10 U of this enzyme and 0.6 μg Hind III digested λDNA- are incubated at 37°C for 4 hours.
RNase residue detection: RNA electrophoresis bands do not change when 10 U of this enzyme and 1 μg of total RNA of HeLa cell are incubated at 37°C for 1 hour.
E.coil DNA residue detection: E.coil Genome residue of 200 U of this product should be less than 10 copies in TaqMan qPCR detection specified with 16s rDNA .
Enzyme inactivation test: DNA electrophoresis bands do not change when the product was treated at 50 ℃ for 10 min and incubated with 1 μg of dU-containing dsDNA template for 30 min.
Recommended reaction mixture for PCR
10×PCR Buffer (Mg2+ Plus)
25 mM MgCl2
dUTP (10 mM)
dCTP / dGTP/ dATP/ dTTP (10 mM each)
0.2 mM each
Primer 1 (10 μM)
Primer 2 (10 μM)
Taq DNA Polymerase (5 U/μL)
Heat-Labile UDG (1 U/μL)
Up to 501
According to the experimental requirements, the final concentration of dUTP can be adjusted between 0.2-0.6 mM. 0.2 mMdTTP can be added selectively
dU-containing template degradation
UDG inactivation, template Pre-denaturation
The reaction time at 25 ℃ can be adjusted within 5-10 min according to the experimental needs.