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Product Information

Product Name

Catalog No.

Size

Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES72

250 U

Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES76

500 U

Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES80

1000 U

Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES92

10 KU

Hieff Unicon® Hotstart Direct Taq DNA Polymerase, 5 U/μL

10717ES93

25 KU

Product Description

Hieff Unicon® Hotstart Direct Taq DNA Polymerase is a hot start DNA polymerase that is resistant to blood and other inhibitors. The product is blocked by antibodies and has good amplification sensitivity and specificity. After heating for 30 seconds at the pre denaturation temperature, the antibody completely inactivated and the DNA polymerase activity was released. The amplification induced by nonspecific annealing of primers could be effectively inhibited by using the hot start Taq enzyme.

Package Information

Component Number

Components

10717ES72 (250 U)

10717ES76 (500 U)

10717ES80 (1000 U)

10717ES92 (10 KU)

10717ES93 (25 KU)

10717

HotStart D-Taq (5 U/μL)

50 μL

100 μL

200 μL

1 mL×2

1 mL×5

Storage

Transport in ice packs,it can be stored at -20℃ for 1 years.

Storage

Transport in ice packs,it can be stored at -20℃ for 1 years.

Protocol Reaction Setup

Components

Volume (μL)

Final Concentration

2×Buffera

25

Primer/Probe mixb

X

0.1 μM-0.5 μM

HotStart D-Taq (5 U/μL)c

1.2

0.12 U/μL

DNA template b

X

0.1-100 ng

ddH2O

up to 50

-

【Notes】

a) It is necessary to prepare the reaction buffer. If a basic buffer (10 ×) is required, catalog NO. 11373ES is recommended.

b) The amount of DNA and the concentration of probes and primers are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental conditions.

c) In order to adapt to the experimental application, please adjust the amount of enzyme.

Thermal cycling protocol (2-Step cycling protocol)

Stage

Temperature

Time

Cycles

Pre-denaturation

95℃

5 min

1

Denaturation

95℃

15 sec

45

Annealing/Extension

60℃a

30 secb

45

【Notes】

a) The reaction temperature was adjusted according to the Tm value of the designed primers.

b) Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.

15221439726

15221439726