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Hieff NGS® DNA Selection Beads

Product SKU

Specifications

Price

12601ES03

1 mL

$45.70

12601ES08

5 mL

$152.24

12601ES56

60 mL

$970.56

12601ES75

450 mL

$4043.00

Introduction

Hieff NGS® DNA Selection Beads are based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection in the preparation of next generation sequencing(NGS) libraries. Hieff NGS® DNA Selection Beads are compatible with various of DNA and RNA library prep protocols reported in the literature. The method is exactly the same as the currently widely used AMPure XP Beads, and the yield and size distribution of the library are highly consistent with AMPure XP Beads.

Storage

The beads can be stored at 2-8℃ for one year, and avoid freezing.

Protocols

1. Preparation

Equilibrated the selection beads at room temperature for at least 30 minutes before use.

2. size selection

The operation flow of size selection is shown in Figure 1, and the protocol is as follows.

Fig 1. The Operation Flow of Size Selection

1)Mix the beads thoroughly by vortexing every time before pipetting.

2)Add the first round of selection beads to the sample refer to table 1, vortex or pipette 10 times to mix.

3)Bind DNA fragments to selection beads for about 5 mins.

4)Separation of beads + DNA fragments from contaminants and discard larger fragments binding on beads.

5)Add the second round of selection beads to the sample refer to table 1, vortex or pipette 10 times to mix.

6)Bind DNA fragments to selection beads for about 5 mins.

7)Centrifuge the tube briefly and place it on magnetic rack. After the solution is clear (about 5 minutes), discard the supernatant.

8)Keep the tube on magnetic rack, add 200 μL of freshly prepared 80% ethanol to rinse the magnetic beads twice, and carefully remove the supernatant.

9)Keep the tube in the magnetic rack, open the lid and dry the selection beads until cracks just appear (about 5 min).

Note:Do not over-dry the selection beads. Over-dried beads with cracks on the surface will significantly decrease elution efficiency.

10)Take the tube out of the magnetic rack, add an appropriate amount of ddH2O (≥20 μL), vortex or pipette gently to mix well, and incubate at room temperature for 5 min.

11)Centrifuge the tube briefly and place it on magnetic rack to separate the magnetic beads and supernatant. After the solution is clear (about 5 minutes), Transfer the supernatant to new tube.

3. Reference Conditions for DNA Size Selection

The calf thymus DNA was fragmented by sonication to prepare a fragment of 100-1000 bp, and the two rounds of size selection were performed according to Table 1. The results were analyzed using Agilent 2100 Bioanalyzer (Figure 2).

Table 1. Reference condition for DNA size selection

Length of DNA fragment

250-350 bp

320-420 bp

450-550 bp

550-700 bp

700-900 bp

800-1000 bp

Ratio of Beads: DNA for the 1st Round

0.80×

0.70×

0.60×

0.55×

0.50×

0.45×

Ratio of Beads: DNA for the 1st Round

0.20×

0.20×

0.20×

0.15×

0.15×

0.15×

(Volume of Agencourt AMPure XP per reaction) = 1.8 x (Reaction Volume)

15221439726

15221439726