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Bst Ⅱ DNA Polymerase

Product SKU

Specifications

Price

12908ES60

1600 U

$86.93

12908ES97

8000 U

$347.86

12908ES98

40000 U

$1413.22

Product Description

Bst Ⅱ DNA Polymerase is derived from Bacillus stearothermophilus DNA Polymerase I. Its 5'-3' exonuclease activity has been removed by genetic engineering, but 5'-3' DNA polymerase activity and strong strand displacement activity are retained. Compared with the wild-type Bst DNA Polymerase, the enzyme has greatly improved the amplification speed, yield, salt tolerance and thermal stability, while increasing the dUTP tolerance, which is very suitable for anti-pollution isothermal amplification reactions, i.e. LAMP.

Package Information

Component

12908ES60(1600 U)

12908ES97(8000 U)

12908ES97(40000 U)

12908-A 10×Bst Buffer

1 mL×2

1 mL×10

1 mL×50

12908-B Bst Ⅱ DNA Polymerase (8 U/μL)

200 μL

1 mL

1 mL×5

10×Bst Buffer contains 80 mM MgSO4, but contains no dNTP and dUTP.

Storage

Store at -20℃ for 3 years. Transport using ice bag.

Unit definition

1 U refers to the amount of enzyme required to react 10 nmol of dNTP into the acid-insoluble precipitate for 30 min at 65°C.

Heat inactivation

85℃, 5 min.

Protocol
Reaction Setup

Component

Volume (μL)

Final Concentration

ddH2O

to 25

-

10×Bst Buffer

2.5

1× (contains 8 mM MgSO4)

dNTP Mix (10 mM each)

3.5

1.4 mM each

dUTP (100 mM) (optional)

0.35

1.4 mM

UDG/UNG (1U/μL) (optional)

1

1 U

DNA

10 ng~1 μg

-

10×Primers

2.5 μL

-

Bst Ⅱ DNA Polymerase(8 U/μL)

0.25~1 μL

0.08~0.32 U/μL

The recommended concentration of MgSO4 is 6-10 mM. 1 × Bst Buffer already contains 8 mM MgSO4.

10 × Primers: 16 µM FIP / BIP, 2 µM F3 / B3, 4 µM Loop F / B each.

The company's dNTP (Cat No. 10124), dUTP (Cat No. 10128) and UDGase (Cat No. 10303) can be used with this product.

Reaction conditions

Temperature

Time

Effect

25~37°C

5~10 min

Degradation of U-containing templates (optional)

65°C

30~60 min

reaction

85℃

5 min

Deactivated

15221439726

15221439726