UltraNuclease is a nonspecific endonuclease recombinant from Serratia Marcescen and expressed in Escherichia coli (E. coli). It catalyzes nonspecific endonucleolytic cleavage of DNA and RNA of all formats (double-stranded, single-stranded, linear, circular, naturally occurring, or denatured) to yield 5'-monophosphate oligonucleotides of 2-5 bp under a broad range of conditions (6 M Urea， 0.1 M Guanidine HCl，0.4% Triton X-100，0.1% SDS，1 mM EDTA，1 mM PMSF). This product has been widely used for nucleic acid digestion for numerous applications.
The product is engineered, expressed from Escherichia coli (E. coli), Purity ≥ 95%. It is often used to reduce cell supernatant or cell lysate viscosity, thus increasing protein purification efficiency and enhancing protein functional research. Besides, the product can also be applied to prevent clumping of human peripheral blood mononuclear cells (PBMC) in cell therapy and vaccine development.
The product is provided in the form of a sterilized reagent, eluted in buffer（20 mM Tris-HCl pH 8.0，2 mM MgCl2，20 mM NaCl，50% glycerin）, with the appearance of colorless, transparent liquid.
Transport with ice packs. Storage at -20℃, Valid for 1 year. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microorganism contamination.
Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
Monovalent Cation Concentration
Phosphate Ion Concentration
UltraNuclease Amount (Final Concentration)
> 1 h
1. Sample Collection
Adherent cells: remove the medium, wash the cells with PBS, and remove the supernatant.
Suspension cells: collect the cells by centrifugation, wash the cells with PBS, centrifuge at 6,000 rpm for 10 min, and collect the pellet.
Escherichia coli: collect the bacteria by centrifugation, wash once with PBS, centrifuge at 8,000 rpm for 5 min, and collect the pellet.
2. Sample Treatment
Treat the collected cell pellets with lysis buffer at the ratio of mass (g) to volume (mL) 1: (10-20), or by mechanical or chemical methods on ice or at room temperature (1 g of cell pellet contains about 109 cells).
3. Enzyme Treatment
Add UltraNuclease according to the ratio of 250 Units to digest 1 g of cell pellets. Please refer to the “Recommended Treatment Time” form above to choose the duration of the treatment.
4. Supernatant Collection
Centrifuge at 12,000 rpm for 30 minutes and collect the supernatant.
Note: If the solution is acidic or alkaline, or contains high concentrations of salt, detergents, or denaturants, please increase the enzyme dosage or extend the treatment time accordingly.