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Product Information

Product Name

Catalog No.

Size

Hieff® anti-Taq DNA Polymerase Antibody

31301ES60

100 µg

Hieff® anti-Taq DNA Polymerase Antibody

31301ES80

1 mg

Hieff® anti-Taq DNA Polymerase Antibody

31301ES90

5 mg

Hieff® anti-Taq DNA Polymerase Antibody

31301ES92

10 mg

Hieff® anti-Taq DNA Polymerase Antibody

31301ES93

100 mg

Hieff® anti-Taq DNA Polymerase Antibody

31301ES94

1000 mg

Product Description

Hieff® anti-Taq DNA Polymerase Antibody is a Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of DNA polymerase after binding with Taq DNA Polymerase. It can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperature. Hieff® anti-Taq DNA Polymerase Antibody denatured in the initial DNA denaturation step of PCR reaction, and the activity of DNA polymerase was restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibody.

Storage

Transport in ice packs,it can be stored at -20℃ for 2 years

Applications

The concentration of this product is 5 mg/mL. It is recommended that 1 μL antibody block the activity of 20-50 U Taq DNA polymerase and mix it at room temperature for 20 min. The blocking efficiency is more than 90%.

Quality Control

Exonuclease Activity: Incubation of 5 µL of this product with 0.6 μg λ-Hind III at 37℃ for 1 hour results in no change on electrophoretic bands.

Endonuclease Activity: Incubation of 5 µL of this product with 0.6 μg Supercoiled pBR322 DNA for 1 hour at 37℃ results in no change on electrophoretic bands.

Mouse Genomic DNA Contamination Test: In 50 µL system, 5 µL of the product was added, and aseptic ddH2O was used as template to amplify mouse actin gene. After 35 cycles, the amplified products were subjected to 1% agarose gel electrophoresis and EB staining without amplification bands.

15221439726

15221439726