The protocol of Ulcerative Colitis Drosophila Modeling using Dextran sodium sulfate (DSS)-Yeasen

Dextran sulfate sodium (DSS) is a polyanionic derivative of dextran produced by the esterification of Dextran with chlorosulphonic acid. Feeding Drosophila will destroy the connection between intestinal epidermal cells in the intestinal epithelium, causing intestinal damage, causing multiple pathways to respond to regulate the self-renewal and differentiation of stem cells, replenish damaged cells, and maintain intestinal homeostasis.

Figure 1. Schematic diagram of division and differentiation of Drosophila intestinal stem cells

Figure 1. Schematic diagram of division and differentiation of Drosophila intestinal stem cells

1. Model building

2. Results

3. Links to this resource

4. Successful case for modeling with Yeasen DSS

5. Product order

6. Published articles with our reagents

7. Regard Reading

1 Model building

1.1 Animal Model

Drosophila, female, 5-10 days;

1.2 Material

DSS(Yeasen#60316ES,MW36000-50000)

1.3 Protocol

a) Feeding medium was prepared with 5% sucrose solution;
b) Control group: 5% sucrose medium (SC), Experimental group: 3% DSS (medium preparation);
c) Flies were cultured in an empty vial containing a piece of 2.5×3.75-cm chromatography paper (Fisher) wet with 5% sucrose solution as feeding medium；
d) Drosophila was cultured at 29°C for three days with a daily change of culture bottle;
e) After three days of culture, the midguts were dissected out and immunostained with GFP (green) and PH3 (red) antibodies and DRAQ5 (blue).

2. Results

Figure 2. The proliferation of ISC precursor cells in Drosophila melanogaster induced by DSS [1]

Figure 2. The proliferation of ISC precursor cells in Drosophila melanogaster induced by DSS ^[1]

By using different labeling methods in immunostaining, the intestinal stem cells (ISC) and the proliferation of ISC precursor cells were quantified, indicating that DSS induced intestinal damage in the drosophila model.

3. Links to this resource

[1] Fangfang Ren,et al.PNAS.2010.107 (49) 21064-21069

4. Successful case for modeling with Yeasen DSS

Yeasen provides high-quality DSS (Cat#60316ES): purity (98%), sulfur content 17-19%, free sulfur <0.2%, supported by a large number of literature data.

Table 1. Construction of different types of enteritis models with DSS

Model	Modeling samples	Modeling plan	Modeling results	Use evaluation
acute colitis	BALB/c mice, female, 6-8 weeks, 25 g	3%-5% DSS drink freely for 7 consecutive days	Day 5 appeared the length of the colon was shortened, HE staining, and the inflammation was obvious	molding speed is fast and the time is short. Consistent with the characteristics of the acute colitis model
acute colitis	C57BL/6 mice, male, 8 weeks, 20 g	3%-5% DSS by gavage, continuous administration	Day 5 occurs, colon shortening, weight loss, blood in the stool, diarrhea	High mold rate, and short duration. Consistent with the characteristics of the acute colitis model
chronic colitis	C57BL/6 mouse, male, 8 weeks, 22 g	1-2% DSS by gavage, continuous administration	Day40 appears, colon shortening, weight loss, blood in the stool, diarrhea	High molding rate. Consistent with the characteristics of the chronic colitis model
colon cancer	C57BL/6 mouse, male, 8 weeks, 21 g	1%-2% DSS ad libitum for 5 days for 3 weeks	14 weeks with shortened colon length, weight loss, HE staining, and obvious inflammation	High molding rate. Consistent with colon cancer model characteristics

5. Product order

Hot-selling product only needs 1/3 of the price of M* with the same efficiency, and we keep a large stock.

Table 2. Product Order

Product Name	Article Number	Specification
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES25	25g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES60	100 g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES76	500 g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES80	1 kg

6. Published articles with our reagents

[1]Li Zhao, Fei Wang, Zhengwei Cai, et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J]. chemical engineering journal.424(2021)130464.IF=16.744
[2]Lingjun Tong, Haining Hao, Zhe Zhang, et al.Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556
[3]Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
[4]Jingjing Gan, Yuxiao Liu, Lingyu Sun, et al.Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J]. Applied Materials Today.2021 Dec;25:101231 IF=10.041
[5]Mengmeng Xu, Ying Kong, Nannan Chen,et al.Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis[J].Frontiers in Immunology.2022; 13: 855645. IF=7.561
[6]JialiDong, YuanLi,HuiwenXiao, et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[7]Hao H, Zhang X, Tong L, Liu Q, et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[8]Yaohua Fan, Yanqun Fan, Kunfeng Liu, et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
[9]Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei, et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[10]Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[11]Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[12] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076