YEASEN ECL Series Luminescence Kit — Light up your imprinted membrane

ECL reagents, also known as horseradish peroxidase (HRP) substrates, are currently the most sensitive reagents for Western blotting detection. At present, common ECL reagents on the market often have some problems such as easy quenching, high background, instability and low abundance protein difficult to detect, which affect the final experimental results.

 

According to the current situation, YEASEN concentrate excellent scientific researchers in the field of immune detection, on the basis of strict control of luminol raw materials, and constantly improve the reinforcer and stabilizer formula, according to different needs to develop 4 kinds of ECL reagents, including Enhanced, Super, SuperDura and MaxiSignal products. It can not only meet the requirements of the experimenter for the detection of the expression of the target protein from low abundance to high abundance, but also improve the luminescence intensity without affecting the experimental conditions, so that you can get a clearer image.

 

 

Excellent sensitivity — can detect pick to low fick grade antigens

Higher signal-to-noise ratio — precise luminescent substrate reduces background

Greatly save antibody — Optimized substrate system, higher antibody binding force

Excellent cost performance — higher performance, lower price

Excellent stability — new oxidant, stable storage at 4℃ for one year

 

Product principle

The core principle of ECL reagent detection is oxidation reaction: Luminol as the main ingredient of luminous substrate, in alkaline conditions,catalyzed by horseradish peroxidase (HRP), it is oxidized by H₂O₂ to produce the excited intermediate of 3-amino-phthalic acid. When it returns to the ground state, it emits a photon with a maximum emission wavelength of 425 nm. The photon signal can be captured by X-ray film or CCD imager.

 

 

Selection guide

At present, YEASEN has 4 kinds of ECL products, each with its own characteristics, which can fully meet users' requirements for western blotting applications.

 

So, which product should you choose for your experiment?

 

Table 1. ECL reagent selection guide

YEASEN	Enhanced ECL	Super ECL	SuperDura	MaxiSignal
Applicable  samples	Target abundance is higher, conventional protein samples	Target abundance is low, limited protein samples	Target abundance is lower,limited protein samples	Target abundance is very low,  precious protein samples
Detection sensitivity	medium pick-grade	low pick-grade	medium fick-grade	low fick-grade
Signal duration	< 2 h	< 12 h	< 24 h	< 8 h
Applicable detection system	All reagents are suitable for X - ray film, CCD imager
Dilution ratio of primary antibody	1:1000-1:4000	1:1000-1:10,000	1:1000-1:50,000	1:5000-1:100,000
Dilution ratio of secondary antibody	1:1000-1:4000	1:2000-1:10,000	1:50,000-1:250,000	1:100,000-1:500,000
Recommend membrane	NC membrane	NC membrane or PVDF membrane	NC membrane or PVDF membrane	NC membrane or PVDF membrane
Storage stability	room temperature for one year	room temperature for one year	room temperature for one year	4℃ for one year, room temperature for half of a year
advantage	The signal is strongest at the same level of sensitivity	The most cost-effective, suitable for most WB experiments	Maximum signal duration, ideal for multiple exposures	Excellent sensitivity, extremely suitable for trace protein detection

Product Name	Cat#	Specifications
Super ECL Detection Reagent	36208ES60	100 mL
	36208ES76	500 mL
Enhanced ECL Chemiluminescent Substrate Kit	36222ES60	100 mL
	36222ES76	500 mL
SuperSignal SuperDura Extended Duration Substrate	36223ES10	10 mL
	36223ES60	100 mL
	36223ES70	200 mL
	36223ES76	500 mL
SuperSignal MaxiSignal Maximum Sensitivity Substrate	36224ES10	10 mL
	36224ES60	100 mL
	36224ES70	200 mL
	36224ES76	500 mL