Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/μL

Details:

Description

Heat-Labile UDG (uracil DNA glycosylase) catalyzes the release of free uracil from uracil-containing DNA by hydrolyzing the N-glycoside bond between uracil bases and sugar-phosphate skeletons. Compared with conventional UDG enzymes, heat-Labile UDG can avoid the degradation of dU-containing amplification products caused by the residual activity of the inactivated UDG at room temperature. Heat-Labile UDG (uracil DNA glycosylase) works at room temperature and is thermolabile and easy to be inactivated.

Applications

  • Study of DNA repair and mutation detection.
  • Increase the cloning efficiency of PCR products.
  • Increase the efficiency of site-directed mutagenesis.

Specifications

Enzyme Glycosylase
Compatible Buffer Storage Buffer
Heat Inactivation 50°C, 10 min
Unit Definition One unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.

Components

Components No. Name 10303ES60 (100 U) 10303ES76 (500 U) 10303ES96 (10,000 U) 10303ES97 (100 mL) 10303ES98 (500 mL)
10303 Uracil DNA Glycosylase (UDG/UNG), heat-labile 100 μL 500 μL 10 mL 100 mL 500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years.

Documents:
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