Heat-Labile UDG (uracil DNA glycosylase) catalyzes the release of free uracil from uracil-containing DNA by hydrolyzing the N-glycoside bond between uracil bases and sugar-phosphate skeletons. Compared with conventional UDG enzymes, heat-Labile UDG can avoid the degradation of dU-containing amplification products caused by the residual activity of the inactivated UDG at room temperature. Heat-Labile UDG (uracil DNA glycosylase) works at room temperature and is thermolabile and easy to be inactivated.
|Compatible Buffer||Storage Buffer|
|Heat Inactivation||50°C, 10 min|
|Unit Definition||One unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.|
|Components No.||Name||10303ES60 (100 U)||10303ES76 (500 U)||10303ES96 (10,000 U)||10303ES97 (100 mL)||10303ES98 (500 mL)|
|10303||Uracil DNA Glycosylase (UDG/UNG), heat-labile||100 μL||500 μL||10 mL||100 mL||500 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years.