Description

Ceturegel™ Matrix High Concentration, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), and nestin, as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors contained in EHS tumors themselves. Ceturegel™ GFR Matrigel is a low-growth factor matrigel obtained after special treatment, which can effectively ensure the needs of some experimental operations that require low-growth factors, such as experiments related to signaling pathways and growth factors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties, and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for cell morphology, biochemical function, migration, Invasion, and gene expression studies.

The three-dimensional culture matrix formed by Matrigel can promote the adherence and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. Ceturegel™ Matrigel can affect gene expression in three-dimensional cultures of murine hepatocytes and human mammary epithelial cells. At the same time, Ceturegel™ Matrigel can be used as a basic scaffold for various tumor cell invasion studies using matrigel, an essential matrix for in vitro and in vivo studies of angiogenesis, and a three-dimensional scaffold for the growth of transplanted tumor cells in in vivo animal models. Ceturegel™ Matrigel also supports the regeneration of peripheral nerves and the differentiation of bovine fallopian tube epithelial cells.

Ceturegel™ Matrix High Concentration, LDEV-Free Matrigel is a sterile product with a concentration of more than 18 mg/mL, which can meet various experimental requirements, including: in vivo tumorigenesis, angiogenesis research, tumor cell migration, 3D cell research, and other applications.

Feature

• High Safety: No LDEV (lactate dehydrogenase elevating virus)
• Multiple Concentration: the concentration range from 8 to 20 mg/ml
• Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse, etc.
• Low Endotoxin: Endotoxin content <8 EU/ml
• Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
• High yield:＞30L per single batch
• Wide Compatibility: Compatible with almost all types of cell culture medium

Application

• 2D Culture Support
• Migration/Invasion
• Biochemical Research
• 3D & Organoid Culture
• Angiogenesis
• In Vivo Tumor Formation

Specification

Concentration	≥18 mg/mL
Product Type	Basement Membrane Matrix
Endotoxin Level	Low
Transportation Conditions	Dry Ice Transportation
Product Line	Ceturegel™
Form	Frozen
Phenol Red Indicator	Contain
Product specifications	5/10 mL
Species	EHS Mouse Tumors
Classification	High Concentration
Serum Level	None
LDEV Detection	None

Components

Components No.	Name	40187ES08	40187ES10
40187	Ceturegel™ Matrix High Concentration, LDEV-Free	5 mL	10 mL

Shipping and Storage

Dry ice shipping. -15℃ ~ -25℃ storage, valid for two years.

Figures

• Cell application validation

Figure 1. The application validation of Ceturegel™ Matrix was as shown.

The representative image of HepG2 cell stained with crystal violet after the invasion (Figure 1A). The image of the 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)