Description

Hieff NGS™ Ultima Pro PCR Free DNA Library Prep Kit V2 is a new generation PCR Free library preparation kit specially developed and designed for the Illumina high-throughput sequencing platform. Based on the previous generation of library preparation kits, this product exhibits higher efficiencies in end repair, dA-tailing, and adapter ligation than the previous versions. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA. 

Features

• Suitable for 5ng-1000 ng of DNA, including FFPE, cfD, NA etc.
• Proven to provide high-quality libraries and sequencing data.
• Strict batch stability.

Applications

• Preparation of DNA library for Illumina platform
• Suitable for sequencinthe g in the tumor, reproductive genetics, genetic disease detection, and other fields
• PCR-free library preparation

Specifications

Product Type	Library Preparation Kit
Libraries	Fragment Library
Fragmentation method	Ultrasonic
Input amount	5 ng - 1000 ng
For Use With (Equipment)	Illumina Platforms
Sample Type	gDNA/FFPE/cfDNA
Sequencing Type	Genome & DNA Sequencing
Product Line	DNA library Preparation
For Use With (Application)	NGS PCR Free library preparation
Quantity	8 /24/96 Reactions

Components

Components No.	Name	12196ES08 (8T)	12196ES24 (24T)	12196ES96 (96T)
12196-A	Endprep Buffer 2.0	48 μL	144 μL	576 μL
12196-B	Endprep Enzyme 2.0	32 μL	96 μL	384 μL
12196-C	Ligation Enhancer 2.0	240 μL	720 μL	3×960 μL
12196-D	Rapid T4 DNA Ligase 2.0	40 μL	120 μL	480 μL

Storage

-25℃ ~ -15℃ storage, valid for one year.

Figures

Figure 1． Comparison of the transformation efficiencies of the PCR-Free libraries

Method: Using ultrasonic interrupted calf gDNA sample as a template, 200 ng and 500 ng was used to build PCR Free library. 50 ng of each library was used for sequencing, and the library conversion efficiency was judged by the amount of data.
Results: The sequencing data volume of 12196 libraries is higher than that of competing products, and the library conversion efficiency is higher than that of competing products.

Figure 2.  Comparison of GC homogeneity in PCR-Free libraries

Method: Using ultrasonic interrupted calf gDNA sample as a template, 200 ng and 500 ng was used to build PCR Free library. 50 ng of each library was used for sequencing.
Results: Compared with competing kits, 12196 sequencing data for different template inputs, the GC homogeneity was better.