Description

Protein A/G MagBeads use "nano-surface biotechnology" (S-TEC) to target Protein A/G onto the surface of superparamagnetic polymer microspheres with high density. It has higher antibody binding ability and very low non-specific adsorption rate of protein. One-step purification can isolate the antibody with purity >90% from the serum sample, which is simple and effective to use. Natural Protein A is a cell wall surface protein found in Staphylococcus aureus. Natural Protein G is a cell surface protein isolated from the genus G or C Streptococcus.  Both have similar functions and bind most mammalian IgG by interacting primarily with the Fc region of immunoglobulin (Ig), but differ in their binding specificity. Protein A/G MagBeads covalently conjugate both protein A and protein G, providing A wider binding range and higher utility than either protein A or protein G alone. At the same time, this product uses genetically modified proteins A and G, which not only maintain their Ig affinity properties, but also remove the non-major binding domain of the natural protein itself to reduce non-specific binding. This product has a wide range of application, can be used in cell lysis fluid, cell secretory fluid supernatant, serum, animal ascites and other immune antigen samples.

Features

• Low background—little to no nonspecific binding, and no preclearing 
• Highly reproducible—uniform beads ensure the most consistent results 
• Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP,  of low abundance proteins 
• Fast and easy— no centrifugation or preclearing steps 
• Versatile—products for IP, Co-IP, pull-down, and ChIP assays.

Applications

• Immunoprecipitation (IP)
• Purification of antibody

Specifications

Ligand	rProtein A/G
Binding Capacity	≥50 μg hIgG /mg
Particle size	1 μm
Concentration	10 mg/mL
Storage Buffer	PBS, 0.01% Tween-20, 0.02% NaN3

Components

Components No.	Name	36417ES03	36417ES08
36417	rProtein A/G MagBeads (IP Grade)	1 mL	5 mL

Storage

The products should be stably stored at 2~8℃ for 2 year.

Figures

Figure 1. The SDS-PAGE gel electrophoresis results of Rabbit serum purification by Protein A/G MagBeads (IP Grade).

L: sample
L: eluate