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Protein A/G MagBeads(IP Grade) 

DetailsDocumentsCitations & ReferencesCOA
Details:

Description

Protein A/G MagBeads use "nano-surface biotechnology" (S-TEC) to target Protein A/G onto the surface of superparamagnetic polymer microspheres with high density. It has higher antibody binding ability and very low non-specific adsorption rate of protein. One-step purification can isolate the antibody with purity >90% from the serum sample, which is simple and effective to use. Natural Protein A is a cell wall surface protein found in Staphylococcus aureus. Natural Protein G is a cell surface protein isolated from the genus G or C Streptococcus.  Both have similar functions and bind most mammalian IgG by interacting primarily with the Fc region of immunoglobulin (Ig), but differ in their binding specificity. Protein A/G MagBeads covalently conjugate both protein A and protein G, providing A wider binding range and higher utility than either protein A or protein G alone. At the same time, this product uses genetically modified proteins A and G, which not only maintain their Ig affinity properties, but also remove the non-major binding domain of the natural protein itself to reduce non-specific binding. This product has a wide range of application, can be used in cell lysis fluid, cell secretory fluid supernatant, serum, animal ascites and other immune antigen samples.

Features

  • Low background—little to no nonspecific binding, and no preclearing 
  • Highly reproducible—uniform beads ensure the most consistent results 
  • Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP,  of low abundance proteins
  • Fast and easy— no centrifugation or preclearing steps
  • Versatile—products for IP, Co-IP, pull-down, and ChIP assays

Applications

  • Immunoprecipitation (IP)
  • Purification of antibody

 

Specifications

Ligand

rProtein A/G

Binding Capacity

≥50 μg Rabbit IgG /mg

Particle size

1 μm

Concentration

10 mg/mL

Storage Buffer

PBS, 0.01% Tween-20, 0.02% NaN3

 

Components

Components No.

Name

36417ES03

36417ES08

36417

rProtein A/G MagBeads (IP Grade)

1 mL

5 mL

 

Shipping and Storage

The products are shipped with ice pack. It can be stably stored at 2~8℃ for 2 year.

 

Figures

Figure 1. The SDS-PAGE gel electrophoresis results of Rabbit serum purification by Protein A/G MagBeads (IP Grade).

L: sample
L: eluate

Documents:
Citations & References:

[1] Zhou L, Zhuang H-J, Chen Q, Jiang L-P, Han X-M, Ge Y-X, et al. Precise targeting of osteopontin in non-small cell lung cancer spinal metastasis to promote chemosensitivity via a smart hollow nano-platform. Chemical Engineering Journal. 2022;436:132131.(IF:15.1)

[2] Jiang Y, Guo H, Tong T, et al. lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. Mol Ther. 2022;30(1):448-467. doi:10.1016/j.ymthe.2021.06.006(IF:11.454)

[3] Wang Y, Gao W-Y, Wang L-L, Wang R-L, Yang Z-X, Luo F-Q, et al. FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination. Clinical and Translational Medicine. 2022;12(7):e891.(IF:10.6)

[4] Wang Y, Gao WY, Wang LL, et al. FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination. Clin Transl Med. 2022;12(7):e891. doi:10.1002/ctm2.891(IF:8.554)

[5] Cen Y, Zou X, Zhong Q, et al. The TIAR-mediated Nrf2 response to oxidative stress is mediated through the Nrf2 noncoding 3'untranslated region in Spodoptera litura. Free Radic Biol Med. 2022;184:17-29. doi:10.1016/j.freeradbiomed.2022.03.016(IF:7.376)

[6] Hua Z, Wei R, Guo M, Lin Z, Yu X, Li X, et al. YTHDF2 promotes multiple myeloma cell proliferation via STAT5A/MAP2K2/p-ERK axis. Oncogene. 2022;41(10):1482-91.(IF:8.0)

[7] Zhou W, He P, Liu H, Wei H, Yu J. A luciferase based automated assay for rapid and sensitive detection of SARS-CoV-2 antibodies. Analytica Chimica Acta. 2023;1238:340633.(IF:6.2)

[8] Zhenzhen L, Wenting L, Jianmin Z, et al. miR-146a-5p/TXNIP axis attenuates intestinal ischemia-reperfusion injury by inhibiting autophagy via the PRKAA/mTOR signaling pathway. Biochem Pharmacol. 2022;197:114839. doi:10.1016/j.bcp.2021.114839(IF:5.858)

[9] Wang L, Yi J, Yin XY, et al. Vacuolating Cytotoxin A Triggers Mitophagy in Helicobacter pylori-Infected Human Gastric Epithelium Cells. Front Oncol. 2022;12:881829. Published 2022 Jul 14. doi:10.3389/fonc.2022.881829(IF:5.738)

[10] Meng J, Zhang C, Wang D, Zhu L, Wang L. Mitochondrial GCN5L1 regulates cytosolic redox state and hepatic gluconeogenesis via glycerol phosphate shuttle GPD2 [published online ahead of print, 2022 Jun 28]. Biochem Biophys Res Commun. 2022;621:1-7. doi:10.1016/j.bbrc.2022.06.092(IF:3.575)

[11] Chen P, Wang L, Long YB, et al. E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori. Insect Sci. 2022;29(4):1006-1016. doi:10.1111/1744-7917.12991(IF:3.262)

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