UCF.ME™ Hotstart Sensitive Taq DNA Polymerase (5 U/μL)

Details:

Product description

Hieff UCF.ME™  Hotstart Sensitive Taq DNA Polymerase is purified from a recombinant strain of E. coliis effectively eliminated the DNA from E. coli and other microbial DNA contamination in the product after handling with the purification process specially developed by YEASEN.It is a hot start DNA polymerase with double blocking by double antibodiesCat. No.31303ES.This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity.The antibody is inactivated after heating for 30 seconds at the pre denaturation temperature, DNA polymerase activity and exonuclease activity can be released.The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation.

Specifications

Source

Recombinant E. coli

Storage Buffer

20mM Tris-HCl, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP-40, 0.5% Tween-20, 50% Glycerol, pH 8.0±0.2 @25℃

Unit Definition

Using activated zebrafish sperm DNA as a template/primer, the activity is defined as 1 unit (U) when incorporating 10 nmol of total nucleotides as acid-insoluble activity within 30 min at 74°C.

Components

Name

14314ES72

250 U

14314ES76

500 U

14314ES80

1 KU

14314ES92

10 KU

14314ES93

25 KU

14314ES98

100 KU

UCF.ME™  Hotstart Sensitive Taq DNA Polymerase (5 U/μL)

50 μL

100 μL

200 μL

2×1 mL

5 mL

20 mL

Storage

This product should be stored at -25~-15℃ for 2 years.

Instructions

  1. The recommended amount to add in a 20 μL system is 5-10 units (U), and the input quantity can be adjusted based on the actual results.
  2. The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.

 Notes

  1. This product is for scientific research purposes only.
  2. For your safety and health, please wear lab coats and disposable gloves for operation.

Ver. EN20240426

 

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