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0TelN Protelomerase (5 U/μL)
Product description
TelN Protelomerase is a recombinant expression from phage N15. It cuts double-stranded DNA (dsDNA) at TelN recognition sequences (56 bp), and generates covalently closed ends at the cleavage sites, which can be applied to enzymatic synthesis of DNA.
Specifications
|
Cat.No. |
14540ES72 /14540ES80 / 14540ES90 |
|
Size |
250U / 1000 U / 5000 U |
|
Unit Definition |
One unit is defined as the amount of enzyme that will convert 0.5 μg of supercoiled plasmid containing telN recognition sites into closed linear dsDNA, in 20 μL reaction system containing 1 X TelN Reaction Buffer at 30℃ for 30 minutes. |
|
Recognition Sites |
TATCAGCACACAATTGCCCATTATACGC↓GCGTATAATGGACTATTGTGTGCTGATA ATAGTCGTGTGTTAACGGGTAATATGCG↑CGCATATTACCTGATAACACACGACTAT |
|
Heat Inactivation |
75℃ for 5 min |
Components
|
Components No. |
Name |
14540ES72 |
14540ES80 |
14540ES90 |
|
14540-A |
TelN Protelomerase (5 U/μL) |
50 μL |
200 μL |
1 mL |
|
14540-B |
10 X TelN Reaction Buffer |
250 μL |
1 mL |
5 X 1 mL |
Storage
This product should be stored at -25~-15℃ for 1 years.
Instructions
1. Reaction system preparation
|
Components |
Volume (μL) |
|
dsDNA (< 300 fmol of TelN sites) |
X |
|
10 X TelN Reaction Buffer |
2 |
|
TelN Protelomerase (5 U/μL) |
1 |
|
Nuclease-free water |
To 20 |
2. Reaction condition: 30℃ for 30 min after mixing well.
3. Heat inactivation: 75℃ for 5 min.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
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