PNGase F

Product description

PNGase F is an amidase enzyme that is Cloned from Peace Station Eliza and mainly secreted by Neisseria meningitidis and other Gram-negative bacteria. Yeasen PNGase F is recombinantly expressed in yeast (specific activity: 100,000 U/mL), which can cleave high mannose, hybrid, and complex oligosaccharide glycoproteins linked by asparagine. The cleavage site of PNGase F is the amide bond between the inner N-acetylglucosamine (GlcNAc) and asparagine residue of glycoproteins, while converting the asparagine on the protein after enzymatic hydrolysis to aspartic acid. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.

Feature

.High purity—No protease, glycosidase contamination, purity ≥95%.

.High Activity— 100,000 U/mL.

.Good compatibility—Can be used denaturing or no-denaturing conditions.

Application

.Glycan Sequencing

.Proteomics

.Glycoprotein Analysis

.Recombinant Glycoprotein Expression

Specification

English synonym	PNGase F; N-Glycosidase F; N-Glycosidase F
Source	Yeast recombinant expression
Molecular Weight	36 kDa
Specific Activity	100,000 U/mL
Storage buffer	20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA, 50% Glycerol
Unit Definition	1 unit of enzyme activity refers to the amount of enzyme required to remove more than 95% of the carbohydrates from 10 μg denatured RNase B in a 10 μL reaction system at 37°C within 1 hour.

Components

Components No.	Name	Component Composition	20407ES01	20407ES02
20407-A	PNGase F	PNGase F	15000 U	75000 U
20407-B1	Buffer 1 (10×)	5% SDS; 400 mM DTT	150 μL	750 μL
20407-B2	Buffer 2 (10×)	200 mM Tris, pH 7.5	300 μL	1500 μL
20407-B3	10% NP-40	10% NP-40 in MilliQ-H2O	300 μL	1500 μL

Storage

The product can be stored at -25 to -15℃ for one year.

Figures

Figure 1. purity ≥95%,as determined by SDS-PAGE   1: Protein Marker(Cat#20350ES) 2: PNGase F