Endo H

Product description

Endo H is a recombinant glycosidase，cloned from the corrugated Streptomyces plicatus, which is capable of cleaving the chitobiose core structure of high mannose and certain hybrid oligosaccharides in N-glycoproteins, thereby removing the N-linked high mannose from glycoproteins.

Yeasen Endo H is recombinantly expressed in yeast, and the storage buffer is free of glycerol, which helps to achieve optimal results in HPLC and mass spectrometry analysis. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins.

Feature

1.High purity—No protease, glycosidase contamination, purity ≥95%.

2.High Activity— 1,000,000 U/mL.

3.Good compatibility—Can be used denaturing or no-denaturing conditions.

Application

1.Glycan Sequencing

2.Proteomics

3.Glycoprotein Analysis

4.Recombinant Glycoprotein Expression

Specification

English synonym	Endo H
Source	Yeast recombinant expression
Molecular Weight*	Theoretical value 29 kDa
Activity	1,000,000 U/mL
Storage buffer	20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA
Unit Definition	One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume.

*Due to the influence of the eukaryotic expression in Pichia pastoris, the molecular weight of the target protein, as shown by SDS-PAGE (non-boiled ), is approximately 60 kDa, while the molecular weight of the target protein, as shown by SDS-PAGE (boiled ), is approximately 29 kDa.

Components

Components No.	Name	20414ES92	20414ES97
20414-A	Endo H	10000 U	50000 U
20414-B1	Buffer 1 (10×)	100 μL	500 μL
20414-B2	Buffer 2 (10×)	200 μL	1000 μL

Storage

The product can be stored at -25 to -15℃ for one year.

Figures

Figure 1. Enzymatic cleavage effect  (substrate) 1: Protein Marker(Cat#20350ES) 2: Competitor Endo H 3: Competitor Endo H  4: Competitor Endo H 5: Competitor Endo H 6: Yeasen Endo H 7: Yeasen Endo H 8: Yeasen Endo H 9: Yeasen Endo H  10: Substrate