Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.
|Polymerase||Bst DNA Polymerase|
|Heat Inactivation||Incubation at 85°C for 5 min.|
|Solution Components||10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.|
|Activity Definition||1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.|
|Components No.||Name||14402ES92 (8,000 U)||14402ES97 (40,000 U)|
|14402-A||Hieff™ Bst Plus DNA Polymerase (40 U/μL)||200 μL||1 mL|
|14402-B||10 ×Hieff™ Bst Plus DNA Polymerase Buffer||1 mL||3×1 mL|
|14402-C||100 mmol/L MgSO4||1 mL||3×1 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years. Please avoid repeated freeze-thaw.