Product Description

Globin mRNA Depletion Probe (Human) is designed to remove human Globin mRNA. It can effectively remove Globin mRNA from adult, infant, and embryonic sources, including HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1, and HBZ. This product is used with Hieff NGS™ MaxUp rRNA Depletion Kit (Human) (Yeasen#12257) to effectively remove rRNA and Globin mRNA from total RNA. This kit is suitable for both intact and degraded RNA samples. The RNA samples after rRNA & Globin mRNA removal can be used for high-throughput sequencing analysis of mRNA and non-coding RNA, which significantly enhance the proportion of valid data in sequencing results, and cDNA synthesis or other downstream applications.

Product Application

Suitable for 100 ng~1 μg total RNA samples human, mouse and rat blood resources, and both intact or partially degraded RNA samples. 

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for one year.

Cautions

1. Please use consumables that are free of RNase contamination and clean the experimental area regularly. It is recommended to use ThermoFisher's RNAZapTM high-efficiency nucleic acid removal spray to remove RNase contamination.

2. The RNA sample should be free from genomic DNA contamination. If gDNA remains in the sample, it should be digested by DNase I and purified before use. 

3. The maximum input volume of RNA sample is 10 μL. If the sample volume is large, it can be concentrated first. 

4. For your safety and health, please wear lab coats and disposable gloves for operation.

5. For research use only!