This product is optimized 10× capping buffer and is efficient for mRNA capping reaction and 5'end labeling reaction. The formulation of the product: 0.5 M Tris-HCl, 50 mM KCl, 10 mM MgCl2, 10 mM DTT, pH 8.0 at 25℃
This product is produced in accordance with GMP regulations and provided in a liquid form.
Application
Components No. | Name | 10666ES03 | 10666ES10 | 10666ES25 | 10666ES76 |
10666 | 10× Capping Buffer GMP-grade | 1 mL | 10 mL | 25 mL | 500 mL |
This product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figure 1. The capping efficiency almost reached to 100% by vaccinia capping system from YEASEN.
Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.
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