Description

This product is optimized 10× capping buffer and is efficient for mRNA capping reaction and 5'end labeling reaction. The formulation of the product: 0.5 M Tris-HCl, 50 mM KCl, 10 mM MgCl2, 10 mM DTT, pH 8.0 at 25℃

This product is produced in accordance with GMP regulations and provided in a liquid form.

Application

• Capping mRNA prior to translation assays/in vitro translation

Components

Components No.	Name	10666ES03	10666ES10	10666ES25	10666ES76
10666	10× Capping Buffer GMP-grade	1 mL	10 mL	25 mL	500 mL

Shipping and Storage

This product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Figures

Figure 1. The capping efficiency almost reached to 100% by vaccinia capping system from YEASEN.

Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.