Description

Hieff NGS™ Complete Adapter Kit for Illumina is designed for use with DNA and RNA library preparation kits to prepare libraries for sequencing on Illumina Platforms. The complete Adapter Kit contains 2 sets. Each set has 48 adapters labelled with 8-nucleotide indexes (barcode) for multiplexed sequencing applications. Set 1 contains DNA Adapters 01-48. All the reagents provided in the kit have strictly quality controlled and functional verification, which ensures the stability and repetitiveness of the library preparation to the greatest extent.

Specifications

Product Type	Adapter Kit
Libraries	DNA or RNA Library
Input DNA amount	100 pg - 1000 ng
Input RNA amount	10 ng-4 μg
Instrument compatibility	Illumina Platforms
Application	NGS library preparation
Quantity	192/768 Reactions

Components

Components No.	Name	13519ES04 (48×4T)	13519ES16 (48×16T)
13519	DNA Adapter 01-48	14 μL each	56 μL each

Storage

-25℃ ~ -15℃ storage, valid for 18 months.

Figures

• The DNA library structure

Figure 1. The DNA library structure

 

• Sequence information

The sequences of DNA Adapter for Illumina:
Universal Adapter：
5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
index Adapter：
5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’
XXXXXXXX1 represent the 8-nucleotide indexes (barcodes), which used in Sample Sheet, are listed in the Table below:

Figure 2. Sequence information