Description

Hieff UNICON™ Hotstart E-Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre-denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.

Features

• High sensitivity
• effectively inhibit the non-specific amplification

Applications

• Routine PCR amplification of DNA fragments
• Hotstart PCR

Specifications

Polymerase	Taq DNA Polymerase
Purity	≥ 95% (SDS-PAGE)
Hot Start	Built-In Hot Start
Reaction Speed	Standard
Exonuclease Activity	5' - 3'

Components

Components No.	Name	10726ES72 (250 U)	10726ES76 (500 U)	10726ES80 (1000 U)	10726ES92 (10000 U)	10726ES93 (25000 U)	10726ES98 (100,000 U)
10726	Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U/μL	50 μL	100 μL	200 μL	2 mL	5 mL	20 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15°C~-25°C for 2 years.