Hieff Clone™ Zero TA Cloning Kit



This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, it has the following advantages: 1) fast, and the connection reaction can be completed within only 1-5 min. 2) High efficiency, no self-connection, the positive cloning rate is close to 100%, and there is no need to set up blue and white spot screening; 3) The operation is simple, and it takes only 15-20 min from connecting to the coating plate. Ice bath, heat shock and 1 hour resuscitation are omitted in the operation process. 4) It can connect up to 5 kb of products.


  • Fast: It takes only 2-5 minutes to complete the ligation reaction.
  • Simple: Only the target fragment needs to be added.
  • Efficient: The positive rate was nearly 100%.


  • Rapid cloning of A-tail PCR products.
  • Rapid sequencing of PCR products after cloning (using M13F / M13R primers).


Product type

Cloning Kit

Antibiotic-resistant Bacteria

Ampicillin (AmpR)

Methods of cloning


Conditions of carriage

Dry ice


Components No.


10907ES20 (20T)

10907ES50 (50T)


pESI-T vector (30 ng/μL)

20 μL

50 μL


1 kb control insert (40 ng/μL)

5 μL

5 μL


10× Enhancer

20 μL

50 μL


The product should be stored at -25°C~-15℃ for 1 year.


Citations & References:

[1] Qiu L, Wang Y, Tang W, et al. Activated Phosphoinositide 3-Kinase δ Syndrome: a Large Pediatric Cohort from a Single Center in China. J Clin Immunol. 2022;42(4):837-850. doi:10.1007/s10875-022-01218-4(IF:8.317)

[2] Gao R, Han T, Xun H, et al. MYB transcription factors GmMYBA2 and GmMYBR function in a feedback loop to control pigmentation of seed coat in soybean. J Exp Bot. 2021;72(12):4401-4418. doi:10.1093/jxb/erab152(IF:6.992)

[3] Liu Y, Lai M, Li S, et al. LS-106, a novel EGFR inhibitor targeting C797S, exhibits antitumor activities both in vitro and in vivo. Cancer Sci. 2022;113(2):709-720. doi:10.1111/cas.15229(IF:6.716)

[4] Yang Z, Li Y, Gao F, et al. MYB21 interacts with MYC2 to control the expression of terpene synthase genes in flowers of Freesia hybrida and Arabidopsis thaliana. J Exp Bot. 2020;71(14):4140-4158. doi:10.1093/jxb/eraa184(IF:5.908)

[5] Xu S, Mo C, Lin J, et al. Loss of ID3 drives papillary thyroid cancer metastasis by targeting E47-mediated epithelial to mesenchymal transition. Cell Death Discov. 2021;7(1):226. Published 2021 Aug 30. doi:10.1038/s41420-021-00614-w(IF:5.241)

[6] Zhu L, Xia C, Wu L, et al. The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma. Cell Commun Signal. 2019;17(1):92. Published 2019 Aug 13. doi:10.1186/s12964-019-0415-6(IF:5.111)

[7] Huang JH, Jiao YH, Li L, Li DW, Li HY, Yang WD. Small RNA analysis of Perna viridis after exposure to Prorocentrum lima, a DSP toxins-producing dinoflagellate. Aquat Toxicol. 2021;239:105950. doi:10.1016/j.aquatox.2021.105950(IF:4.964)

[8] Li Y, Shan X, Gao R, et al. MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida. Commun Biol. 2020;3(1):396. Published 2020 Jul 27. doi:10.1038/s42003-020-01134-6(IF:4.165)

[9] Li Y, Shan X, Tong L, et al. The Conserved and Particular Roles of the R2R3-MYB Regulator FhPAP1 from Freesia hybrida in Flower Anthocyanin Biosynthesis. Plant Cell Physiol. 2020;61(7):1365-1380. doi:10.1093/pcp/pcaa065(IF:4.062)

[10] Bao T, Shadrack K, Yang S, et al. Functional Characterization of Terpene Synthases Accounting for the Volatilized-Terpene Heterogeneity in Lathyrus odoratus Cultivar Flowers. Plant Cell Physiol. 2020;61(10):1733-1749. doi:10.1093/pcp/pcaa100(IF:4.062)

[11] Guo L, Zhang M, Gao Y, et al. A gonadal mosaicism novel KMT2D mutation identified by haplotype construction and clone sequencing strategy. Clin Chim Acta. 2022;531:197-203. doi:10.1016/j.cca.2022.04.008(IF:3.786)

[12] Liu Q, Zhang L, Shu Z, Ding Y, Tang XM, Zhao XD. Two paternal mosaicism of mutation in ELANE causing severe congenital neutropenia exhibit normal neutrophil morphology and ROS production. Clin Immunol. 2019;203:53-58. doi:10.1016/j.clim.2019.04.008(IF:3.548)

[13] Li GY, Liu XJ, Fang GQ, Yang J, Zhan S, Li MW. Functional characterization of a low-density lipoprotein receptor in the lepidopteran model, Bombyx mori [published online ahead of print, 2022 Apr 11]. Insect Sci. 2022;10.1111/1744-7917.13018. doi:10.1111/1744-7917.13018(IF:3.262)

[14] Chen L, Ren Y, Han K, Stoeck T, Jiang J, Pan H. Re-description and molecular phylogeny of the free-swimming peritrichs Hastatella radians Erlanger, 1890 and H. aesculacantha Jarocki & Jakubowska, 1927 (Ciliophora, Peritrichia) from China. Eur J Protistol. 2022;84:125891. doi:10.1016/j.ejop.2022.125891(IF:3.020)

[15] Zhuang L, Ji Y, Tian P, et al. Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2. BMC Vet Res. 2019;15(1):30. Published 2019 Jan 17. doi:10.1186/s12917-019-1774-3(IF:1.792)

COA Please fill out the COA application form

Catalog No.:*


phone Number:*