Description

2×Hieff™ Canace™ Plus PCR Master Mix (With Dye) is a ready-to-use 2×premixed solution containing Hieff™ Canace™ Plus High-Fidelity DNA Polymerase, dNTPs, and optimized buffer. Two monoclonal antibodies at room temperature that inhibit polymerase activity and 3'→5' exonuclease activity are added to the master mix for easily and highly specific Hot Start PCR. The extension factor is added to the master mix to give the enzyme a long fragment amplification capacity, the length of the amplification t can be up to 13 kb, the enzyme has a 5'→3' DNA polymerase activity and a 3'→5' exonuclease activity, its fidelity is 83 times that of Taq DNA polymerase, which is 9 times that of ordinary Pfu DNA polymerase. It is suitable for the amplification of complex templates, the amplification product is a blunt end.

2×Hieff™ Canace™ Plus PCR Master Mix (With Dye) has the advantages of being fast and easy, high sensitivity, strong specificity, good stability, etc., the reaction system only needs to add primers and templates, and can be amplified by a two-step protocol, simplifying the experimental steps and saving time. This product contains electrophoresis indicator dyes, and PCR products can be used directly for electrophoresis. In addition, the product also contains a specific protective agent, so that the master mix can maintain stable activity after repeated freeze-thaw.

Features

• The Mix contains an electrophoresis indicator dye, which can be used directly after the reaction and is easy to use.
• Accuracy: The fidelity is 83 times higher than that of Taq DNA polymerase and 9 times higher than that of ordinary Pfu DNA polymerase.

Applications

• High fidelity PCR

Specifications

Product specification	Master Mix
Concentration	2×
Hot Start	Built-in Hot Start
Overhang	Blunt
Reaction speed	Rapid
Size (Final Product)	Up to 13kb
Conditions for transportation	Dry ice
Product Type	High-fidelity PCR premixes

Components

Components No.	Name	10154ES01	10154ES03	10154ES08
10154	2×Hieff™ Canace™ Plus PCR Master Mix (With Dye)	100 μL	1 mL	5×1mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -25℃ ~ -15℃ for 1 year.