This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, it has the following advantages: 1) fast, and the connection reaction can be completed within only 1-5 min. 2) High efficiency, no self-connection, the positive cloning rate is close to 100%, and there is no need to set up blue and white spot screening; 3) The operation is simple, and it takes only 15-20 min from connecting to the coating plate. Ice bath, heat shock and 1 hour resuscitation are omitted in the operation process. 4) It can connect up to 5 kb of products.
Product type | Cloning Kit |
Antibiotic-resistant Bacteria | Ampicillin (AmpR) |
Methods of cloning | TOPO™-Blunt |
Conditions of carriage | Dry ice |
Components No. | Name | 10909ES20 (20T) |
10909-A | pESI-Blunt vector (30 ng/μL) | 20 μL |
10909-B | 1 kb control insert (40 ng/μL) | 5 μL |
10909-C | 10× Enhancer | 20 μL |
The product should be stored at -25°C~-15℃ for 1 year.
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