Hieff Clone™ Zero Blunt Cloning Kit



This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, it has the following advantages: 1) fast, and the connection reaction can be completed within only 1-5 min. 2) High efficiency, no self-connection, the positive cloning rate is close to 100%, and there is no need to set up blue and white spot screening; 3) The operation is simple, and it takes only 15-20 min from connecting to the coating plate. Ice bath, heat shock and 1 hour resuscitation are omitted in the operation process. 4) It can connect up to 5 kb of products.


  • Fast: It takes only 2-5 minutes to complete the ligation reaction.
  • Simple: Only the target fragment needs to be added.
  • ​​​​​​​Efficient: The positive rate was nearly 100%.


  • ​​​​​​​Rapid cloning of blunt end PCR products.
  • ​​​​​​​​​​​​​​Rapid sequencing of PCR products after cloning (using M13F / M13R primers).


Product type

Cloning Kit

Antibiotic-resistant Bacteria

Ampicillin (AmpR)

Methods of cloning


Conditions of carriage

Dry ice


Components No.


10909ES20 (20T)


pESI-T vector (30 ng/μL)

20 μL


1 kb control insert (40 ng/μL)

5 μL


10× Enhancer

20 μL


The product should be stored at -25°C~-15℃ for 1 year.


Citations & References:

[1] Tao R, Wang Y, Jiao Y, et al. Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells [published online ahead of print, 2022 Jun 10]. Nucleic Acids Res. 2022;50(11):6423-6434. doi:10.1093/nar/gkac506(IF:19.160)

[2] Tao R, Wang Y, Hu Y, et al. WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing. Signal Transduct Target Ther. 2022;7(1):108. Published 2022 Apr 20. doi:10.1038/s41392-022-00936-w(IF:18.187)

[3] Yang S, Wang N, Kimani S, et al. Characterization of Terpene synthase variation in flowers of wild aquilegia species from Northeastern Asia [published online ahead of print, 2022 Jan 18]. Hortic Res. 2022;9:uhab020. doi:10.1093/hr/uhab020(IF:6.793)

[4] Yu P, Zhou L, Yang WT, et al. Comparative mitogenome analyses uncover mitogenome features and phylogenetic implications of the subfamily Cobitinae. BMC Genomics. 2021;22(1):50. Published 2021 Jan 14. doi:10.1186/s12864-020-07360-w(IF:3.969)

[5] Liu G, Liu Y, Niu B, et al. Genetic mutation of TRPV2 induces anxiety by decreasing GABA-B R2 expression in hippocampus. Biochem Biophys Res Commun. 2022;620:135-142. doi:10.1016/j.bbrc.2022.06.079(IF:3.575)

[6] Li GY, Liu XJ, Fang GQ, Yang J, Zhan S, Li MW. Functional characterization of a low-density lipoprotein receptor in the lepidopteran model, Bombyx mori [published online ahead of print, 2022 Apr 11]. Insect Sci. 2022;10.1111/1744-7917.13018. doi:10.1111/1744-7917.13018(IF:3.262)

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