This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, it has the following advantages: 1) fast, and the connection reaction can be completed within only 1-5 min. 2) High efficiency, no self-connection, the positive cloning rate is close to 100%, and there is no need to set up blue and white spot screening; 3) The operation is simple, and it takes only 15-20 min from connecting to the coating plate. Ice bath, heat shock and 1 hour resuscitation are omitted in the operation process. 4) It can connect up to 5 kb of products.
|Product type||Cloning Kit|
|Antibiotic-resistant Bacteria||Ampicillin (AmpR)|
|Methods of cloning||TOPO™-Blunt|
|Conditions of carriage||Dry ice|
|Components No.||Name||10909ES20 (20T)|
|10909-A||pESI-Blunt vector (30 ng/μL)||20 μL|
|10909-B||1 kb control insert (40 ng/μL)||5 μL|
|10909-C||10× Enhancer||20 μL|
The product should be stored at -25°C~-15℃ for 1 year.
 Tao R, Wang Y, Jiao Y, et al. Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells [published online ahead of print, 2022 Jun 10]. Nucleic Acids Res. 2022;50(11):6423-6434. doi:10.1093/nar/gkac506(IF:19.160)
 Tao R, Wang Y, Hu Y, et al. WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing. Signal Transduct Target Ther. 2022;7(1):108. Published 2022 Apr 20. doi:10.1038/s41392-022-00936-w(IF:18.187)
 Yang S, Wang N, Kimani S, et al. Characterization of Terpene synthase variation in flowers of wild aquilegia species from Northeastern Asia [published online ahead of print, 2022 Jan 18]. Hortic Res. 2022;9:uhab020. doi:10.1093/hr/uhab020(IF:6.793)
 Yu P, Zhou L, Yang WT, et al. Comparative mitogenome analyses uncover mitogenome features and phylogenetic implications of the subfamily Cobitinae. BMC Genomics. 2021;22(1):50. Published 2021 Jan 14. doi:10.1186/s12864-020-07360-w(IF:3.969)
 Liu G, Liu Y, Niu B, et al. Genetic mutation of TRPV2 induces anxiety by decreasing GABA-B R2 expression in hippocampus. Biochem Biophys Res Commun. 2022;620:135-142. doi:10.1016/j.bbrc.2022.06.079(IF:3.575)
 Li GY, Liu XJ, Fang GQ, Yang J, Zhan S, Li MW. Functional characterization of a low-density lipoprotein receptor in the lepidopteran model, Bombyx mori [published online ahead of print, 2022 Apr 11]. Insect Sci. 2022;10.1111/1744-7917.13018. doi:10.1111/1744-7917.13018(IF:3.262)