CTP Solution GMP-grade (100 mM)

Details:

Description

CTP, Cytidine-5'-triphosphate, can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, and the synthesis of choline and ethanolamine.
This product is a transparent colorless aqueous solution prepared from CTP trisodium salt, and free from DNase, RNase and phosphatase contamination.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

  • Validated, product-specific process and analytical methods
  • Product-specific stability
  • Documentation follows applicable GMP guidelines
  • AOF production process and raw materials (TSE & BSE) 
  • Nitrosamine statement
  • Regulatory support documents available
  • Large-scale production
  • Nucleotide in the multiple salt form (Na+, Tris etc) always available to meet different downstream application needs

Application

  • RNA synthesis and amplification
  • Building block for in vitro transcription

Specification

CAS No 65-47-4 (free acid)
Formula C9H13N3Na3O14P3
Molecular Weight 549.10 g/moL
Purity(HPLC) ≥ 99%
Content 100 mM ± 3 mM
Structure

Component

Components No. Name 10130ES03 10130ES08 10130ES25 10130ES76
10130 CTP Solution GMP-grade (100 mM) 1 mL 5 mL 25 mL 500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.

Figures

  • Standard RNA Synthesis

Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.

  • Capped RNA Synthesis

Figure 2. Synthesis of capped RNA in vitro.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.

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