Description

GTP, Guanosine-5'-triphosphate, can be used in various of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, GTP plays an important role in signal transduction as a phosphate or pyrophosphate messenger: GTP can activate G proteins, which can induce multiple protein kinase mediated cascade reations, causing a variety of cytological behaviors, such as cell proliferation, differentiation, etc. GTP can also be used as a high-energy precursor of single nucleotides to participate in the synthesis of DNase and RNase.

This product is a transparent colorless aqueous solution prepared with GTP tris solution, and free of DNase and RNase contamination.

This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature   

• Validated, product-specific process and analytical methods
• Product-specific stability
• Documentation follows applicable GMP guidelines
• AOF production process and raw materials (TSE & BSE) 
• Nitrosamine statement
• Regulatory support documents available
• Large-scale production
• Nucleotide in the multiple salt form（Na⁺, Tris etc) always available to meet different downstream application needs

Application

• RNA synthesis and amplification
• Building block for in vitro transcription

Specification

CAS No	86-01-1 (free acid); 36051-31-7 (3Na salt)
Formula	C10H13N5Na3O14P3 (free acid)
Molecular Weight	589.13 g/moL (free acid)
Purity (HPLC)	≥ 99%
Content	100 mM ± 3 mM
Structure

Component

Components No.	Name	10132ES03	10132ES08	10132ES25	10132ES76
10132	CTP Solution GMP-grade (100 mM)	1 mL	5 mL	25 mL	500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.

Figures

• Standard RNA Synthesis

Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.

• Capped RNA Synthesis

Figure 2. Synthesis of capped RNA in vitro.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.