Hieff NGS™ Complete Adapter Kit for Illumina is designed for use with DNA and RNA library preparation kits to prepare libraries for sequencing on Illumina Platforms. The complete Adapter Kit contains 2 sets. Each set has 48 adapters lablabeledth 8-nucleotide indexes (barcode) for multiplexed sequencing applications. Set 2 contains DNA Adapters 49-96. All the reagents provided in the kit have strictly quality controlled and functional verification, which ensures the stability and repetitiveness of the library preparation to the greatest extent.
Product Type | Adapter Kit |
Libraries | DNA or RNA Library |
Input DNA amount | 100 pg - 1000 ng |
Input RNA amount | 10 ng-4 μg |
Instrument compatibility | Illumina Platforms |
Application | NGS library preparation |
Quantity | 192/768 Reactions |
Components No. | Name | 13520ES04 (48×4T) | 13520ES16 (48×16T) |
13520 | DNA Adapter 49-96 | 14 μL each | 56 μL each |
-25℃ ~ -15℃ storage, valid for 18 months.
Figure 1. The DNA library structure
The sequences of DNA Adapter for Illumina:
Universal Adapter:
5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
index Adapter:
5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’
XXXXXXXX1 represents the 8-nucleotide indexes (barcodes), which are used in Sample Sheet, and are listed in the Table below:
Figure 2. Sequence information
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