Universal TaqMan multiplex qPCR master mix

Multiplex PCR refers to a type of PCR that allows two or more DNA fragments simultaneously amplified in a single reaction tube. Universal TaqMan multiplex qPCR master mix from Yeasen is a 2x Taq master mix that enables up to four fluorescent quantitative PCR reactions in a single reaction well. Yeasen multiplex qPCR mix is supplied with two components, 2× B-Taq buffer and B-Taq HS (5 U/μL). The 2× B-Taq buffer is an optimized multi-reaction buffer that can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. The B-Taq HS is a genetically modified hot-start Taq enzyme with improved amplification sensitivity and specificity.

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Product Description

This product is a 2× Mix pre-mixed reagent that enables up to four fluorescent quantitative PCR reactions in a single reaction well. This product contains the genetically modified antibody method to hot-start Taq enzyme, greatly improving the amplification sensitivity and specificity. At the same time, this product has deeply optimized the multi-reaction buffer, which can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.

Cautions

About Operation

1. Please ensure that the product is completely thawed and thoroughly mixed before use, briefly centrifuge to collect to the bottom of the tube, and place on ice for later use.

2. To avoid cross-contamination between samples and aerosol contamination, it is recommended to prepare the reaction system in a super clean bench.

3. Avoid repeated freezing and thawing of the product during use.

4. For your safety and health, please wear lab coats and disposable gloves for operation.

Experimental Method

a)      Primer design

1.      Primers must be specific.

2.      The Tm value of the primers must be 58-60°C, and the Tm difference of each primer group should be controlled within 1-2°C.

3.      The length of primers is generally controlled between 15-30 bp.

b)      Probe Design

1.      The Tm value of TaqMan probes is approximately 10°C higher (68-70°C) than the corresponding primers.

2.      Inhibit dimerization between TaqMan probes and primers.