Hieff UniconTM Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an antibody and has high amplification sensitivity and specificity. The antibody can be completely inactivated by heating for 30 seconds at a denaturation temperature and the activity of DNA polymerase is restored. Use of the "hot start" Taq DNA Polymerase can effectively inhibit the amplification caused by nonspecific annealing of PCR primers.
Component Number |
Component |
Cat#/Size |
|||||
10717ES72 (250 U) |
10717ES76 (500 U) |
10717ES80 (1000 U) |
10717ES92 (10 KU) |
10717ES93 (25 KU) |
10717ES94 (50 KU) |
||
10717 |
Hotstart D-Taq (5 U/μL) |
50 μL |
100 μL |
200 μL |
2 × 1 mL |
5 × 1 mL |
10 mL |
The Hieff UniconTM Hotstart Direct Taq DNA Polymerase products are shipped with ice pack and can be stored at -20°C for 2 years.
Components |
Vloume(μL) |
Final Concentration |
2× Buffera |
25 |
1× |
Primer/Probe mixb |
X |
0.1 μmol/L-0.5 μmol/L |
Hotstart D-Taq (5 U/μL)c |
1.2 |
0.12 U/μL |
Template DNA |
X |
0.1-100 ng |
Water, nuclease-free |
up to 50 |
- |
[Note]:
(a) According to the different experimental requirements, you need to prepare your own corresponding reaction Buffer. If basic reaction Buffer is required, Cat#11374 is recommended.
(b/d) The amount of DNA and primer concentration in the table above, both are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.
(c) Adjust the amount of Taq enzyme according to the specific experimental application.
Cycle step |
Temperature |
Time |
Cycles |
Initial denaturation |
95℃ |
5 min |
1 |
Denaturation Annealing / Extension |
95℃ 60℃a |
15 sec 30 secb |
45 |
[Note]:
a) Amplification reaction: The amplification reaction temperature is adjusted according to the designed primer Tm values.
b) Fluorescent signal acquisition: Different qPCR instruments require different fluorescence signal acquisition time, please set according to the minimum time limit.
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. This product is for research use ONLY!
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